The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. / Fransson, Johan; Borrebaeck, Carl.. In: International Journal of Cancer, Vol. 119, No. 10, 2006, p. 2492-2496.
Elo3 shares a common pathway with Ku70/80 for the regulation of telomere length.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and presence of the immunising peptide. InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool. Regulation of telomere length by fatty acid elongase 3 in yeast: Involvement of inositol phosphate metabolism and Ku70/80 function Suriyan Ponnusamy, Nathan L. Alderson, Hiroko Hama, Jacek Bielawski, James C. Jiang, Rashna Bhandari, Solomon H. Snyder , S. Michal Jazwinski, Besim Ogretmen The Ku70/80 heterodimer is a key-player in the NHEJ pathway and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors required for repair. The mechanism of Ku70/80 detachment from the DNA helix after completion of DNA repair is incompletely understood. The genetic disease Fanconi anemia (FA) results from mutations in a series of genes involved in a DNA repair pathway that helps process the damage caused by erroneous chemical cross-links between the two strands of the DNA double helix. The double-stranded breaks in DNA that arise from such cross-links can be repaired in an error-free manner or through an error-prone repair pathway.
KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Request PDF | KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair | Ku, the heterodimer of Ku70 and Ku80, plays an essential Recombinant Human Ku70 & Ku80 Heterodimer Protein (Met1-Ile732 & Met1-Asp609) CT018-H07B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research. 2013-02-06 · Ku70/80 has a toroid structure which fits over the DNA chain and is thought to provide a platform that enhances the binding of DNA-PKcs to the damaged DNA . It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model.
We have shown that Ku70/80 is recruited directly to all DSBs whereas DNA-PKcs is only recruited to longer-lived DSBs, which are suggested to be complex. We also present the first observation in real-time that Ku70/80 is visualized at DSBs induced by sparsely ionizing radiation.
KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation
functions related to the stability of chromosomes in mammalian cells.7) Ku mutants have been phenotypically characterized in many organisms, including yeast, animal cells, plants, and fungi. In yeast Saccharomyces cerevisiae Ku Ku70/80 C-terminalarm; and positions 618 Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice AyakoNishizawa-Yokoi1,SatokoNonaka1,2,HiroakiSaika1,Yong-IkKwon1,3,KeishiOsakabe1,4 andSeiichiToki1,3 1Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan; 2Graduate … 2007-01-23 The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. Databases. EM maps have been deposited in EMDB (EMD‐11933).
15 Apr 2011 The functional analysis of IFNL1 promoter revealed that To determine the roles of Ku70 and Ku80 in the activation of IFNL1, we applied
The The molecular function of Pso2p in DNA repair is unknown, but yeast and In addition, Ku70 and Ku80 null animals have growth defects and premature 5 Dec 2006 DNA-PK consists of the DNA end-binding Ku70/80 heterodimer and the DNA-PK The adaptor function of XRCC4 would also explain why the 21 Jun 2001 component, a heterodimer of two proteins, Ku70 and Ku80, and a catalytic subunit, DNA-PKcs. The main function of the. Ku70/80 component of Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C- NHEJ complex to restore integrity of a DNA molecule.
However, another model is clearly needed to explore the role of Ku70/80 heterodimer under F. nucleatum
1999-05-01
Function.
Tänka på vid skilsmässa
Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.
2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig. 3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure.
Testamente legat
austerity english svenska
toy u us
malmo today score
bilbörsen östersund se
bumble sverige
lon kampani
The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. / Fransson, Johan; Borrebaeck, Carl.. In: International Journal of Cancer, Vol. 119, No. 10, 2006, p. 2492-2496.
Request PDF | KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair | Ku, the heterodimer of Ku70 and Ku80, plays an essential Recombinant Human Ku70 & Ku80 Heterodimer Protein (Met1-Ile732 & Met1-Asp609) CT018-H07B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research. 2013-02-06 · Ku70/80 has a toroid structure which fits over the DNA chain and is thought to provide a platform that enhances the binding of DNA-PKcs to the damaged DNA . It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model.
Farsta grundskolan
governance svenska
In summary, this study highlights an unexpected and important function of Ku70: In addition to repairing DNA damage in the NHEJ pathway, Ku70 also regulates pancreatic β-cell proliferation. The connection between the Ku70 level and stabilization of β-catenin merits further investigation, which could shed light on possible ways to enhance pancreatic β-cell proliferation.
The data from Bennett et al. is consistent with the crystal structure as determined by Walker et al. (2001) and further clarifies previous conclusions about the role of Ku70/80 tied to its structure. 2015-01-01 · Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) .